Application of Phase-contrast X-ray Microtomography to Study the Internal Structures of Rhodnius Prolixus

The PhC-SR-μCT is a nondestructive technique that allows the microanatomical investigations and 3D images reconstructions within a short time. This technique performed in blood sucker, Rhodnius prolixus – one of the most important primary vectors of of Trypanosoma cruzi, ethiologic agent of Chagas’ disease in Latin America and also the most well-know studied insect in terms of both physiology and vector-parasite interactions. However, little is known about the development and structure of its internal organs. The aim of this work is to provide a non-invasive option for studying the internal structures of the main vector of Chagas’ disease, which should help to answer important questions concerning anatomy, development, structure and plasticity of insect in general. Three-dimensional rendering images can provide a detailed knowledge of the interior of the insect, which is crucial for a better understanding of its function and evolution.